Indirect evidence for the presence of ribonucleic acid in vesicular stomatitis virions.

نویسندگان

  • H M CHAMSY
  • P D COOPER
چکیده

Concentration of 5 gamma g(ml or less of 5-Auorodeoxyuridine (FDC) and of 5-bromodeoxyridine (BDU) inhibited the growth of adenovirus type 5 and of vaccinia virus in ERK and chick ell1bryo cells, provided that adequate time was allowed for the inhibitors to act. With the same proviso, FD {T and BDU also inhibited the growth of ERK cells. Cnder similar conditions of cultivation, concentrations of up to 100 gamma g(ml of Fnu and of BDU had no effect on the growth of either the New Jersey or the Indiana serotype of vesicular stomatitis virus (VSV) in either ERK or chick emhryo cells. 1 t is concluded that virus particles of both serotypes of VSV contain RNA ratller than DNA.

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منابع مشابه

Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.

The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymer...

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Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid.

Vesicular stomatitis virus (VSV)-specific messenger ribonucleic acid (mRNA) species contain sequences of adenylate-rich RNA which are more heterogeneous in their migration through sodium dodecyl sulfate-polyacrylamide gels than the corresponding fractions from HeLa cell mRNA. VSV virion RNA contains no adenylaterich sequences. The possible role of such sequences in the mRNA species of a cytopla...

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عنوان ژورنال:
  • Virology

دوره 20  شماره 

صفحات  -

تاریخ انتشار 1963