Indirect evidence for the presence of ribonucleic acid in vesicular stomatitis virions.
نویسندگان
چکیده
Concentration of 5 gamma g(ml or less of 5-Auorodeoxyuridine (FDC) and of 5-bromodeoxyridine (BDU) inhibited the growth of adenovirus type 5 and of vaccinia virus in ERK and chick ell1bryo cells, provided that adequate time was allowed for the inhibitors to act. With the same proviso, FD {T and BDU also inhibited the growth of ERK cells. Cnder similar conditions of cultivation, concentrations of up to 100 gamma g(ml of Fnu and of BDU had no effect on the growth of either the New Jersey or the Indiana serotype of vesicular stomatitis virus (VSV) in either ERK or chick emhryo cells. 1 t is concluded that virus particles of both serotypes of VSV contain RNA ratller than DNA.
منابع مشابه
Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.
The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymer...
متن کاملRibonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion.
The virions of vesicular stomatitis virus contain an enzyme that catalyzes the incorporation of ribonucleotides into RNA. The product of the reaction is mainly RNA complementary in base sequence to that of vesicular stomatitis virus RNA.
متن کاملNucleotide composition of the ribonucleic acid of rabies virus.
The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus.
متن کاملAdenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid.
Vesicular stomatitis virus (VSV)-specific messenger ribonucleic acid (mRNA) species contain sequences of adenylate-rich RNA which are more heterogeneous in their migration through sodium dodecyl sulfate-polyacrylamide gels than the corresponding fractions from HeLa cell mRNA. VSV virion RNA contains no adenylaterich sequences. The possible role of such sequences in the mRNA species of a cytopla...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Virology
دوره 20 شماره
صفحات -
تاریخ انتشار 1963